Process / pipelineElectrophysiology
Patch-Clamp Electrophysiology
Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.
Open in MethodMindSoonVideoSoon
Read the full method
Members only
Sign inSign in with a free account to read this section.
Sources
- Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802. DOI: 10.1038/260799a0 ↗
- Hamill, O. P., Marty, A., Neher, E., Sakmann, B., & Sigworth, F. J. (1981). Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Archiv, 391(2), 85-100. DOI: 10.1007/BF00656997 ↗