Process / pipelineBiochemistry
Scatchard Plot Analysis
Scatchard analysis is a graphical method for determining ligand-receptor binding affinity (Kd) and binding capacity (Bmax) from binding data. Developed by George Scatchard in 1949, the Scatchard plot linearizes hyperbolic binding curves, enabling visual detection of multiple binding sites and quantitative parameter estimation.
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Sources
- Scatchard, G. (1949). The attractions of proteins for small molecules and ions. Annals of the New York Academy of Sciences, 51(4), 660-672. DOI: 10.1111/j.1749-6632.1949.tb29487.x ↗
- Rosenthal, H. E. (1967). A graphic method for the determination and presentation of binding parameters in a complex system. Analytical Biochemistry, 20(3), 525-532. DOI: 10.1016/0003-2697(67)90365-2 ↗