方法对比

	时间序列通路富集分析	多组学通路富集分析
领域	生物信息学	生物信息学
方法族	Process / pipeline	Process / pipeline
起源年份	2005–2014	2014–2016 (multi-omics extension of enrichment methods established ~2005)
提出者	Bar-Joseph and colleagues (temporal gene expression); extended by Cheng, Bhatt et al. for pathway-level time-series inference	Building on Subramanian et al. (2005); multi-omics integration formalised by Meng et al. and others (~2014–2016)
类型	Functional enrichment analysis with temporal modeling	Integrative pathway analysis pipeline
开创性文献	Ernst, J., Nau, G. J., & Bar-Joseph, Z. (2005). Clustering short time series gene expression data. Bioinformatics, 21(Suppl 1), i159–i168.	Meng, C., Kuster, B., Culhane, A. C., & Gholami, A. M. (2014). A multivariate approach to the integration of multi-omics datasets. BMC Bioinformatics, 15, 162.
别名	temporal pathway analysis, longitudinal pathway enrichment, dynamic pathway analysis, TPEA	multi-omics pathway analysis, integrated pathway enrichment, multi-layer pathway enrichment, MOPEA
相关	5	1
摘要	Time-series pathway enrichment analysis identifies biological pathways whose coordinated gene activity changes significantly across ordered time points. Rather than treating each time point independently, the method models the temporal trajectory of gene expression within each pathway and tests whether entire biological programs — not just individual genes — are activated or suppressed in a time-dependent manner. It is widely used in developmental biology, drug response studies, and infection time courses.	Multi-omics pathway enrichment analysis is a bioinformatics pipeline that integrates molecular data from two or more omics layers — such as transcriptomics, proteomics, metabolomics, and epigenomics — and tests whether the combined signal from those layers converges on specific biological pathways more than expected by chance. By considering multiple molecular levels simultaneously, it identifies pathway-level dysregulation that single-omics analyses would miss.