方法对比
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| 蛋白质组学分析× | 序列比对× | |
|---|---|---|
| 领域 | 生物信息学 | 生物信息学 |
| 方法族 | Process / pipeline | Process / pipeline |
| 起源年份≠ | 1994–2003 (term coined 1994; shotgun proteomics established early 2000s) | 1970 (global alignment); 1981 (local alignment) |
| 提出者≠ | Marc Wilkins, Matthias Mann, Ruedi Aebersold (proteome/mass spectrometry foundations) | Saul B. Needleman & Christian D. Wunsch (global); Temple F. Smith & Michael S. Waterman (local) |
| 类型≠ | Quantitative omics pipeline | Computational sequence analysis technique |
| 开创性文献≠ | Wilkins, M. R., Sanchez, J.-C., Gooley, A. A., Appel, R. D., Humphery-Smith, I., Hochstrasser, D. F., & Williams, K. L. (1996). Progress with proteome projects: Why all proteins expressed by a genome should be identified and how to do it. Biotechnology and Genetic Engineering Reviews, 13(1), 19–50. link ↗ | Needleman, S. B., & Wunsch, C. D. (1970). A general method applicable to the search for similarities in the amino acid sequence of two proteins. Journal of Molecular Biology, 48(3), 443–453. DOI ↗ |
| 别名 | proteomics, mass spectrometry-based proteomics, shotgun proteomics, quantitative proteomics | pairwise alignment, multiple sequence alignment, MSA, sequence comparison |
| 相关 | 6 | 6 |
| 摘要≠ | Proteomics analysis is a systematic pipeline for identifying and quantifying proteins in biological samples using mass spectrometry. Starting from raw spectral data, the workflow searches protein sequence databases, estimates abundance across conditions, applies statistical tests for differential expression, and maps findings onto biological pathways. It complements transcriptomics by capturing post-translational regulation and actual protein abundance, and is central to biomarker discovery, drug-target identification, and systems biology. | Sequence alignment is a foundational bioinformatics technique that arranges two or more DNA, RNA, or protein sequences to reveal regions of similarity, infer evolutionary relationships, identify functional domains, and map sequencing reads to reference genomes. It underpins virtually every downstream genomic analysis, from variant calling and gene expression quantification to phylogenetics and structural annotation. |
| ScholarGate数据集 ↗ |
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