Process / pipelineFunctional genomics
CRISPR筛选分析
CRISPR筛选分析处理来自CRISPR-Cas9的群体遗传筛选数据，以识别在特定条件下细胞生长、存活或表型所必需的基因。该计算流程由张锋、Sanjana等人开发，将向导RNA丰度的测序读数转化为功能基因的排名列表。
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来源
- Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., ... & Zhang, F. (2014). Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 343(6166), 84-87. DOI: 10.1126/science.1247005 ↗
- Hart, T., Chandrashekhar, M., Aregger, M., Steinhart, Z., Brown, K. R., MacLeod, G., ... & Moffat, J. (2015). High-resolution CRISPR screens reveal fitness genes and pathways. Molecular Systems Biology, 11(8), 820. link ↗
- King, J. B., Palmer, A. C., & Sorger, P. K. (2020). Application of a genetic algorithm designed for flexible objective optimization in pharmaceutical research and development. Cancer Research, 79(13 Supplement), 3435. link ↗
如何引用本页
ScholarGate. (2026, June 3). CRISPR Screening Data Analysis and Hit Identification. ScholarGate. https://scholargate.app/zh/bioinformatics/crispr-screen-analysis
Which method?
Set this method beside its closest kin and read them side by side — the library lays the books on the table; the choice is yours.
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