Uporedite metode

	Analiza raznolikosti mikrobioma korišćenjem više omika	Analiza obogaćenja genskih skupova (GSEA)
Oblast	Bioinformatika	Bioinformatika
Porodica	Process / pipeline	Process / pipeline
Godina nastanka	2010s–present	2005 (seminal PNAS paper; predecessor concept in Mootha et al. 2003)
Tvorac	Developed collectively; key frameworks by Le Cao et al. (mixOmics, 2017) and Argelaguet et al. (MOFA, 2018)	Aravind Subramanian, Pablo Tamayo, Vamsi K. Mootha, Jill P. Mesirov, Todd R. Golub, Eric S. Lander et al. (Broad Institute)
Tip	Integrative computational pipeline	Functional genomics / enrichment analysis
Temeljni izvor	Rohart, F., Gautier, B., Singh, A., & Le Cao, K.-A. (2017). mixOmics: An R package for 'omics feature selection and multiple data integration. PLOS Computational Biology, 13(11), e1005752.	Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550.
Drugi nazivi	multi-omics microbiome profiling, integrated microbiome omics, multi-modal microbiome analysis, microbiome multi-omics integration	GSEA, gene-set analysis, functional enrichment analysis, pathway-level enrichment
Srodne	5	5
Sažetak	Multi-omics microbiome diversity analysis integrates two or more omic data layers — such as metagenomics, metatranscriptomics, metabolomics, and metaproteomics — to characterise both the composition and functional activity of microbial communities. By linking taxonomic diversity metrics with molecular phenotype data, the approach uncovers how community structure translates into ecological and host-relevant functions that no single omic layer can reveal alone.	Gene Set Enrichment Analysis (GSEA) is a computational method that determines whether a predefined set of genes — representing a biological pathway, process, or function — shows statistically significant, coordinated differences between two biological conditions. Unlike simple fold-change filtering, GSEA operates on all measured genes ranked by a correlation metric, detecting subtle but consistent shifts across an entire pathway even when no single gene passes a significance threshold.