Salīdzināt metodes
Apskatiet izvēlētās metodes blakus; rindas, kas atšķiras, ir izceltas.
| Analīze CRISPR skrīningiem× | Metagenomiskā iedalīšana× | |
|---|---|---|
| Nozare | Bioinformātika | Bioinformātika |
| Saime | Process / pipeline | Process / pipeline |
| Izcelsmes gads≠ | 2013 | 2011 |
| Autors≠ | Feng Zhang | Jillian Banfield |
| Tips≠ | High-throughput genetic screen pipeline | Sequence assembly and clustering pipeline |
| Pirmavots≠ | Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., ... & Zhang, F. (2014). Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 343(6166), 84-87. DOI ↗ | Kang, D. D., Froula, J., Egan, R., & Wang, Z. (2015). MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities. PeerJ, 3, e1165. DOI ↗ |
| Citi nosaukumi≠ | CRISPR pooled screen, genetic screen analysis | metagenomic assembly, genome binning, MAG recovery |
| Saistītās | 3 | 3 |
| Kopsavilkums≠ | CRISPR screen analysis processes data from pooled genetic screens using CRISPR-Cas9 to identify genes required for cell growth, survival, or phenotype in specific conditions. Developed by Zhang, Sanjana, and others, this computational pipeline transforms sequencing readouts of guide RNA abundances into ranked lists of functional genes. | Metagenomic binning partitions assembled contigs from complex microbial communities into distinct genome bins, each representing an individual organism or strain. Pioneered by Banfield and colleagues, this pipeline isolates single-organism genomes (metagenome-assembled genomes or MAGs) from environmental samples without requiring cultivated isolates. |
| ScholarGateDatu kopa ↗ |
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