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용혈 시험 (Hemolysis Assay)×CAM 분석×
분야생체재료학생체재료학
계열Process / pipelineProcess / pipeline
기원 연도19501974
창시자Clinical hematology traditionsJudah Folkman
유형Hemolytic compatibility assayDevelopmental biology assay
원전ASTM F756-17 (2017). Standard Practice for Assessment of Hemolytic Properties of Materials. ASTM International. link ↗Folkman, J. (1974). Tumor angiogenesis: therapeutic implications. New England Journal of Medicine, 285(21), 1182-1186. link ↗
별칭RBC lysis assay, hemolytic compatibility test, hemolytic potential testchick embryo chorioallantoic membrane, angiogenesis assay, CAM angiogenesis model
관련44
요약The hemolysis assay is a standard method for evaluating the blood compatibility of biomaterials by quantifying the extent to which a material or substance damages red blood cells (RBCs) and causes hemoglobin release. Codified in standards including ASTM F756 and ISO 10993-4, the hemolysis assay is essential for regulatory approval of blood-contacting devices such as stents, catheters, artificial heart valves, and hemodialysis membranes. The assay provides a simple, quantitative measure of hemolytic potential that correlates with clinical safety.The chorioallantoic membrane (CAM) assay is a well-established in vivo model for studying angiogenesis (new blood vessel formation) and evaluating the pro- or anti-angiogenic properties of biomaterials, drugs, and bioactive molecules. Developed by Judah Folkman in the 1970s, the assay uses the highly vascularized CAM of developing chick embryos as a platform for implanting test materials and observing vascular response. The CAM provides a transparent, immunologically naive microenvironment with rapid and reproducible neovascularization, making it ideal for screening angiogenic potential and assessing biomaterial biocompatibility.
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