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	メタゲノムビニング	CRISPRスクリーニング解析
分野	バイオインフォマティクス	バイオインフォマティクス
系統	Process / pipeline	Process / pipeline
提唱年	2011	2013
提唱者	Jillian Banfield	Feng Zhang
種類	Sequence assembly and clustering pipeline	High-throughput genetic screen pipeline
原典	Kang, D. D., Froula, J., Egan, R., & Wang, Z. (2015). MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities. PeerJ, 3, e1165.	Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., ... & Zhang, F. (2014). Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 343(6166), 84-87.
別名	metagenomic assembly, genome binning, MAG recovery	CRISPR pooled screen, genetic screen analysis
関連	3	3
概要	Metagenomic binning partitions assembled contigs from complex microbial communities into distinct genome bins, each representing an individual organism or strain. Pioneered by Banfield and colleagues, this pipeline isolates single-organism genomes (metagenome-assembled genomes or MAGs) from environmental samples without requiring cultivated isolates.	CRISPR screen analysis processes data from pooled genetic screens using CRISPR-Cas9 to identify genes required for cell growth, survival, or phenotype in specific conditions. Developed by Zhang, Sanjana, and others, this computational pipeline transforms sequencing readouts of guide RNA abundances into ranked lists of functional genes.