Process / pipelineMolecular ecology

eDNA Metabarcoding

Environmental DNA (eDNA) metabarcoding detects and identifies species present in environmental samples (water, soil, air) by sequencing short DNA fragments released by organisms. Developed by Taberlet and colleagues (2012), this approach has revolutionized biodiversity monitoring: species can be surveyed without capture, observation, or complex sampling designs. Metabarcoding sequences millions of DNA fragments, identifies reads taxonomically, and assigns them to species. The method is non-invasive, rapid, and cost-effective, enabling large-scale biodiversity surveys and early detection of cryptic or rare species.

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Sources

  1. Taberlet, P., Coissac, E., Hajibabaei, M., & Rieseberg, L. H. (2012). Environmental DNA. Molecular Ecology, 21(8), 1789-1793. DOI: 10.1111/j.1365-294X.2012.05542.x
  2. Deakin, G., Pettitt-Wade, H., & Waldick, R. C. (2016). Environmental DNA metabarcoding: A review of the application to fish biodiversity assessment in temperate freshwaters. Environmental DNA, 1(1), 4-14. DOI: 10.1002/edn3.3
  3. Ficetola, G. F., Miaud, C., Pompanon, F., & Taberlet, P. (2008). Species detection using environmental DNA from water samples. Biology Letters, 4(4), 423-425. DOI: 10.1098/rsbl.2008.0118

Related methods

ScholarGateeDNA Metabarcoding (Environmental DNA Metabarcoding). Retrieved 2026-06-04 from https://scholargate.app/en/ecology/edna-metabarcoding