Rhizosphere Amplicon Analysis — Root-Zone Microbiome Profiling

Rhizosphere Amplicon Analysis is a molecular-ecological pipeline used to characterise the microbial communities inhabiting the root-adjacent soil zone — the rhizosphere — by sequencing targeted marker genes such as the bacterial 16S rRNA gene or the fungal ITS region. Widely applied in agronomy, soil ecology, and plant pathology, it enables researchers to identify which microorganisms are present, how their composition shifts under different crops, treatments, or soil conditions, and how community structure relates to plant health and productivity.