Process / pipelineQuantification Strategy
Standard Addition Method
The standard addition method is a quantitative analytical technique that determines the concentration of an unknown analyte by measuring the response before and after adding a known quantity of the analyte (a standard) to the sample itself. This approach compensates for matrix effects and interference from other sample components, making it invaluable when analyzing complex matrices (biological fluids, environmental samples, geological materials) where the sample composition profoundly affects the measured signal. The standard addition method is widely recognized in analytical chemistry as a primary quantification strategy when external calibration is compromised by matrix variability.
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Sources
- Harris, D. C. (2010). Quantitative Chemical Analysis (8th ed.). Freeman. ISBN: 978-1429218153
- Ellison, S. L. R., & Barwick, V. J. (2000). Estimating measurement uncertainty: reconciliation using a phylogenetic approach. Accreditation and Quality Assurance, 5(4), 205–213. DOI: 10.1007/s007690050419 ↗
- Kochmann, S., Lobera, M. P., Carrera, C., Muller, N., & Guilland, J. C. (2012). Determination of selenium in serum and whole blood by inductively coupled plasma mass spectrometry. Clinical Chemistry and Laboratory Medicine, 50(8), 1371–1377. DOI: 10.1515/cclm-2012-0049 ↗