So sánh phương pháp

	Phân tích RNA-seq đơn bào dựa trên mạng lưới	Phân tích làm giàu đường dẫn
Lĩnh vực	Tin sinh học	Tin sinh học
Họ	Process / pipeline	Process / pipeline
Năm ra đời	2015–2017 (rapid development alongside scRNA-seq methods; SCENIC 2017)	2003–2005
Người khởi xướng	Aibar et al. (SCENIC, gene regulatory networks); Jin et al. (CellChat, cell-cell communication networks)	Mootha et al. (2003); systematised by Subramanian et al. (2005)
Loại	Computational bioinformatics pipeline	Statistical functional annotation method
Công trình gốc	Aibar, S., González-Blas, C. B., Moerman, T., Huynh-Thu, V. A., Imrichova, H., Hulselmans, G., ... & Aerts, S. (2017). SCENIC: single-cell regulatory network inference and clustering. Nature Methods, 14(11), 1083–1086.	Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550.
Tên gọi khác	scRNA-seq network analysis, single-cell gene regulatory network inference, scGRN analysis, single-cell co-expression network analysis	PEA, overrepresentation analysis, ORA, functional enrichment analysis
Liên quan	6	6
Tóm tắt	Network-based single-cell RNA-seq analysis extends standard scRNA-seq workflows by constructing and interrogating molecular interaction networks — gene regulatory networks, co-expression networks, or cell-cell communication graphs — from single-cell transcriptomic data. Rather than treating each gene independently, this approach captures the coordinated activity of gene circuits and intercellular signalling pathways within and between cell populations, enabling a systems-level view of transcriptional regulation at single-cell resolution.	Pathway enrichment analysis (PEA) is a statistical approach that takes a list of genes or proteins of interest — typically derived from a differential expression or proteomics experiment — and identifies which pre-defined biological pathways or functional gene sets are represented more often than expected by chance. By mapping individual molecular changes onto curated pathway knowledge bases such as KEGG, Gene Ontology, or Reactome, PEA translates long gene lists into interpretable biological processes, making it a central tool in the post-analysis of high-throughput omics experiments.