เปรียบเทียบวิธี

	การเรียกหาตำแหน่งสูงสุดของ ChIP-seq ระดับเซลล์เดี่ยว	การวิเคราะห์ Single-cell RNA-seq
สาขาวิชา	ชีวสารสนเทศศาสตร์	ชีวสารสนเทศศาสตร์
ตระกูล	Process / pipeline	Process / pipeline
ปีกำเนิด	2019	2009 (first scRNA-seq by Tang et al.); widely adopted 2015–2016
ผู้ริเริ่ม	Grosselin et al.; Ku et al. (parallel independent development)	Azim Surani, Barbara Treutlein, and the Regev/McCarroll groups (foundational droplet-based methods ~2015)
ประเภท	Epigenomic profiling pipeline	High-throughput single-cell transcriptomic profiling pipeline
แหล่งต้นตำรับ	Grosselin, K., Durand, A., Marsolier, J., Poitou, A., Marangoni, E., Nemati, F., ... & Vallot, C. (2019). High-throughput single-cell ChIP-seq identifies heterogeneity of chromatin states in breast cancer. Nature Genetics, 51(6), 1060-1066.	Satija, R., Farrell, J. A., Gennert, D., Schier, A. F., & Regev, A. (2015). Spatial reconstruction of single-cell gene expression data. Nature Biotechnology, 33(5), 495–502.
ชื่อเรียกอื่น	scChIP-seq peak calling, single-cell chromatin profiling, scChIC-seq analysis, single-cell epigenomic peak detection	scRNA-seq, single-cell transcriptomics, scRNAseq analysis, single-cell gene expression profiling
ที่เกี่ยวข้อง	5	5
สรุป	Single-cell ChIP-seq peak calling is a bioinformatics pipeline that identifies genomic regions enriched for histone modifications or transcription factor binding in individual cells. By profiling chromatin states at single-cell resolution, it reveals epigenomic heterogeneity hidden in bulk ChIP-seq experiments, enabling researchers to map regulatory landscapes across distinct cell populations within a complex tissue sample.	Single-cell RNA sequencing (scRNA-seq) analysis characterises gene expression at the resolution of individual cells, enabling discovery of cell types, states, and transitions that are invisible in bulk transcriptomics. Starting from raw sequencing reads, the workflow produces a cell-by-gene count matrix and proceeds through quality control, normalisation, dimensionality reduction, unsupervised clustering, cell-type annotation, and a range of downstream analyses such as trajectory inference and differential expression between cell populations.