Uporedite metode

	Mrežna analiza metaboloma	Analiza obogaćenja genskih skupova (GSEA)
Oblast	Bioinformatika	Bioinformatika
Porodica	Process / pipeline	Process / pipeline
Godina nastanka	2005–2011	2005 (seminal PNAS paper; predecessor concept in Mootha et al. 2003)
Tvorac	Barabasi, Loscalzo and colleagues (network medicine framework); Wishart and Xia (metabolomics network tools)	Aravind Subramanian, Pablo Tamayo, Vamsi K. Mootha, Jill P. Mesirov, Todd R. Golub, Eric S. Lander et al. (Broad Institute)
Tip	Systems biology / omics analysis pipeline	Functional genomics / enrichment analysis
Temeljni izvor	Xia, J., & Wishart, D. S. (2010). MSEA: a web-based tool to identify biologically meaningful patterns in quantitative metabolomic data. Nucleic Acids Research, 38(Web Server issue), W71–W77.	Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550.
Drugi nazivi	metabolic network analysis, systems metabolomics, network metabolomics, metabolite network enrichment	GSEA, gene-set analysis, functional enrichment analysis, pathway-level enrichment
Srodne	6	5
Sažetak	Network-based metabolomics analysis integrates quantitative metabolite profiling data with biological network structures — metabolic pathways, protein-metabolite interaction graphs, and disease networks — to reveal coordinated biochemical disruptions that individual metabolite lists would miss. Rather than treating each metabolite in isolation, this systems-level approach identifies modules, hubs, and perturbed subnetworks, providing mechanistic insight into how metabolic dysregulation propagates through cellular systems.	Gene Set Enrichment Analysis (GSEA) is a computational method that determines whether a predefined set of genes — representing a biological pathway, process, or function — shows statistically significant, coordinated differences between two biological conditions. Unlike simple fold-change filtering, GSEA operates on all measured genes ranked by a correlation metric, detecting subtle but consistent shifts across an entire pathway even when no single gene passes a significance threshold.