Сравнение методов
Просматривайте выбранные методы рядом; строки с различиями подсвечены.
| Де ново сборка транскриптома× | Анализ CRISPR-скрининга× | |
|---|---|---|
| Область | Биоинформатика | Биоинформатика |
| Семейство | Process / pipeline | Process / pipeline |
| Год появления≠ | 2011 | 2013 |
| Автор метода≠ | Aviv Regev | Feng Zhang |
| Тип≠ | Sequence assembly pipeline | High-throughput genetic screen pipeline |
| Основополагающий источник≠ | Grabherr, M. G., Haas, B. J., Yassour, M., Levin, J. Z., Thompson, D. A., Amit, I., ... & Regev, A. (2011). Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 29(7), 644-652. DOI ↗ | Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., ... & Zhang, F. (2014). Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 343(6166), 84-87. DOI ↗ |
| Другие названия≠ | transcriptome assembly, de novo assembly, RNA-Seq assembly | CRISPR pooled screen, genetic screen analysis |
| Связанные | 3 | 3 |
| Сводка≠ | De novo transcriptome assembly reconstructs full-length messenger RNA sequences directly from sequencing reads without requiring a reference genome. Pioneered by Regev, Haas, and colleagues, this pipeline enables transcript discovery in non-model organisms and detection of novel isoforms, fusion genes, and splice variants. | CRISPR screen analysis processes data from pooled genetic screens using CRISPR-Cas9 to identify genes required for cell growth, survival, or phenotype in specific conditions. Developed by Zhang, Sanjana, and others, this computational pipeline transforms sequencing readouts of guide RNA abundances into ranked lists of functional genes. |
| ScholarGateНабор данных ↗ |
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