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표준 첨가법×UV-Vis 분광광도법×
분야분석화학분석화학
계열Process / pipelineProcess / pipeline
기원 연도1920s1852
창시자Analytical chemistry practiceAugust Beer
유형matrix-compensating calibration techniqueabsorption measurement technique
원전Harris, D. C. (2010). Quantitative Chemical Analysis (8th ed.). Freeman. ISBN: 978-1429218153Beer, A. (1852). Bestimmung der Absorption des rothen Lichts in farbigen Flussigkeiten. Annalen der Physik und Chemie, 86(5), 78–88. DOI ↗
별칭spiking method, known-addition method, matrix matchingUV-Vis spectroscopy, absorption spectroscopy, colorimetry
관련55
요약The standard addition method is a quantitative analytical technique that determines the concentration of an unknown analyte by measuring the response before and after adding a known quantity of the analyte (a standard) to the sample itself. This approach compensates for matrix effects and interference from other sample components, making it invaluable when analyzing complex matrices (biological fluids, environmental samples, geological materials) where the sample composition profoundly affects the measured signal. The standard addition method is widely recognized in analytical chemistry as a primary quantification strategy when external calibration is compromised by matrix variability.UV-Vis spectrophotometry is an optical analytical technique that measures the absorption of ultraviolet and visible light (wavelengths 190–900 nm) by substances in solution. Founded on the Beer-Lambert law (developed by August Beer and Pierre Bouguer), it is one of the oldest and most widely used quantitative analytical methods. UV-Vis spectrophotometry is economical, rapid, and applicable to a vast range of organic and inorganic compounds, making it indispensable in pharmaceutical, clinical, environmental, and research laboratories.
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