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| 스크래치 상처 분석법× | 생존/사멸 분석× | |
|---|---|---|
| 분야 | 생체재료학 | 생체재료학 |
| 계열 | Process / pipeline | Process / pipeline |
| 기원 연도≠ | 2007 | 2000 |
| 창시자≠ | Liang, Park, and Guan | Invitrogen/Molecular Probes |
| 유형≠ | Migration assessment | Dual-dye viability assay |
| 원전≠ | Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333. DOI ↗ | Molecular Probes (2004). LIVE/DEAD Viability/Cytotoxicity Kit user guide. Invitrogen Corporation. link ↗ |
| 별칭 | wound healing assay, gap closure assay, migration assay | calcein-AM/propidium iodide, SYTO/PI staining, fluorescent viability stain |
| 관련 | 4 | 4 |
| 요약≠ | The scratch wound assay (also called the wound healing assay or gap closure assay) is a simple, cost-effective method for measuring cell migration in vitro. Developed and standardized by Liang, Park, and Guan in 2007, the assay involves creating a defined gap (wound) in a monolayer of confluent cells using a pipette tip or specialized tool, then monitoring the rate at which cells migrate into the gap over hours to days. The scratch wound assay is widely used to evaluate the effects of growth factors, inhibitory compounds, and biomaterial extracts on cell motility and wound healing potential. | The Live/Dead assay is a fluorescence-based method for simultaneously identifying live and dead cells using two complementary dyes. The assay combines calcein-AM (or SYTO fluorophores), which generates bright green fluorescence in living cells with intact esterase activity, with propidium iodide (PI), which produces red fluorescence in dead cells with compromised membrane integrity. Commercially developed by Molecular Probes and now part of Thermo Fisher's portfolio, the Live/Dead kit is widely used to evaluate cell viability on biomaterial scaffolds, in tissue constructs, and following drug or toxin exposure. |
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