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패치 클램프 전기생리학×쉴트 분석×
분야약리학약리학
계열Process / pipelineProcess / pipeline
기원 연도19761947
창시자Erwin Neher and Bert SakmannHenry Schild
유형ion channel screeningantagonism quantification
원전Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802. DOI ↗Schild, H. O. (1947). pA, a new scale for the measurement of drug antagonism. Journal of Physiology, 106(3), 337-357. DOI ↗
별칭patch clamp, whole-cell recording, ion channel assaySchild plot, pA2
관련33
요약Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.Schild analysis is a quantitative method for characterizing competitive receptor antagonism developed by Henry Schild in 1947. It uses dose-response curves in the presence and absence of antagonist to estimate the antagonist affinity constant (pA2), enabling standardized comparison of antagonist potency across drugs and experimental systems.
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ScholarGate방법 비교: Patch-Clamp · Schild Analysis. 2026-06-15에 다음에서 검색함: https://scholargate.app/ko/compare