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패치 클램프 전기생리학×Michaelis-Menten 동역학×
분야약리학약리학
계열Process / pipelineProcess / pipeline
기원 연도19761913
창시자Erwin Neher and Bert SakmannLeonor Michaelis and Maud Menten
유형ion channel screeningmechanistic model
원전Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802. DOI ↗Michaelis, L., & Menten, M. L. (1913). Die Kinetik der Invertinwirkung. Biochemische Zeitschrift, 49, 333-369. link ↗
별칭patch clamp, whole-cell recording, ion channel assayMM kinetics, Michaelis constant, Vmax
관련32
요약Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.Michaelis-Menten kinetics describes the rate of enzyme-catalyzed reactions as a function of substrate concentration. Developed by Leonor Michaelis and Maud Menten in 1913, this foundational framework models enzyme catalysis through the rapid-equilibrium approximation and enables prediction of drug metabolism rates in pharmacokinetics.
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