手法を比較

	Caco-2細胞透過性アッセイ	f1/f2 類似度因子（Dissolution f1/f2 Similarity Factor）
分野	薬理学	薬理学
系統	Process / pipeline	Process / pipeline
提唱年	1989	1996
提唱者	Ingrid Hidalgo	James Moore and Hector Flanner
種類	absorption screening	similarity testing
原典	Hidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749.	Moore, J. W., & Flanner, H. H. (1996). Mathematical comparison of dissolution profiles. Pharmaceutical Technology, 20(6), 64-74.
別名	Caco-2 assay, intestinal permeability, ADME screening	f1, f2, similarity factor
関連	3	3
概要	The Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport.	The f1 and f2 factors are dimensionless statistical measures developed by Moore and Flanner to quantify the similarity between two dissolution profiles. Adopted by regulatory agencies (FDA, EMA) as the gold standard for comparing dissolution curves, these factors enable rapid assessment of whether formulation changes significantly impact drug release.