Usporedite metode

	Analiza protočnom citometrijom	Test propusnosti stanica Caco-2	Patch-Clamp Elektrofiziologija
Područje	Farmakologija	Farmakologija	Farmakologija
Obitelj	Process / pipeline	Process / pipeline	Process / pipeline
Godina nastanka	1976	1989	1976
Tvorac	Leonard Herzenberg	Ingrid Hidalgo	Erwin Neher and Bert Sakmann
Vrsta	cell analysis and sorting	absorption screening	ion channel screening
Temeljni izvor	Herzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M., & Herzenberg, L. A. (2002). The history and future of the fluorescence-activated cell sorter and flow cytometry: a view from Stanford. Clinical Chemistry, 48(10), 1819-1827.	Hidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749.	Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802.
Drugi nazivi	FACS, fluorescence-activated cell sorting, cell analysis	Caco-2 assay, intestinal permeability, ADME screening	patch clamp, whole-cell recording, ion channel assay
Srodne	3	3	3
Sažetak	Flow cytometry is a laser-based technology for analyzing and sorting individual cells based on fluorescent markers. Developed by Leonard Herzenberg in the 1970s, flow cytometry enables rapid assessment of cell phenotype, drug effects on cell populations, and therapeutic cell characterization in immunology and hematology.	The Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport.	Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.