השוואת שיטות

	ניתוח העשרת קבוצות גנים בסדרות עתיות	ניתוח העשרת מסלולים
תחום	ביואינפורמטיקה	ביואינפורמטיקה
משפחה	Process / pipeline	Process / pipeline
שנת המקור	2005 (GSEA foundation); time-series adaptations 2007–2014	2003–2005
הוגה השיטה	Extension of GSEA (Subramanian et al., 2005); time-series adaptations developed through maSigPro (Conesa lab) and related tools	Mootha et al. (2003); systematised by Subramanian et al. (2005)
סוג	Gene set enrichment method for longitudinal omics data	Statistical functional annotation method
מקור מכונן	Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550.	Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550.
כינויים	longitudinal GSEA, dynamic GSEA, time-course GSEA, TS-GSEA	PEA, overrepresentation analysis, ORA, functional enrichment analysis
קשורות	6	6
תקציר	Time-series gene set enrichment analysis (TS-GSEA) extends the classical GSEA framework to detect biologically coordinated gene sets — pathways, gene ontology terms, or curated signatures — whose collective expression changes meaningfully over time. Rather than comparing two snapshots, it models the full temporal trajectory of gene expression to identify which functional programs are activated, suppressed, or dynamically remodelled during a biological process such as development, treatment response, or disease progression.	Pathway enrichment analysis (PEA) is a statistical approach that takes a list of genes or proteins of interest — typically derived from a differential expression or proteomics experiment — and identifies which pre-defined biological pathways or functional gene sets are represented more often than expected by chance. By mapping individual molecular changes onto curated pathway knowledge bases such as KEGG, Gene Ontology, or Reactome, PEA translates long gene lists into interpretable biological processes, making it a central tool in the post-analysis of high-throughput omics experiments.