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MTT/MTS Assay/Evidence
Method evidence record

MTT/MTS Assay

The MTT assay, introduced by Tatsuro Mosmann in 1983, is a colorimetric method for quantifying cell viability and proliferation by measuring mitochondrial metabolic activity. The method detects the conversion of the water-soluble tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by active mitochondria, producing an insoluble purple formazan precipitate proportional to the number of viable cells. The related MTS assay, which does not require solubilization, offers improved kinetics and is now widely adopted in both academic research and pharmaceutical development.

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Source record

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MTT/MTS Cell Viability and Proliferation Assay
Taxonomic method record · process-pipeline / biomaterials
  • Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63. · DOI 10.1016/0022-1759(83)90303-4
  • Slade, P. G. (1999). MTS tetrazolium compound (abstract). Methods in Cell Biology, 63, 65-72. · URL
  • Riss, T. L., Moravec, R. A., Niles, A. L., et al. (2004). Cell viability assays. In Assay Guidance Manual. Eli Lilly & Company and the National Center for Advancing Translational Sciences. · URL
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Related methods

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Same method familyElectrospinningmachine-suggested · Relational suggestion, not evidence.Same method familyHemolysis Assaymachine-suggested · Relational suggestion, not evidence.Same method familyLive/Dead Assaymachine-suggested · Relational suggestion, not evidence.Same method familyScratch Wound Assaymachine-suggested · Relational suggestion, not evidence.

Evidence status

Sources recorded, not reviewed

Bibliographic sources are present. Claim-level evidence review has not been performed.

Sources

3 recorded citations, copied from the method source record.

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