Process / pipelineBioinformatics / omics
ChIP-seq Peak Calling — Chromatin Immunoprecipitation Sequencing Peak Calling
ChIP-seq peak calling is a computational pipeline that identifies genomic regions where a protein of interest — a transcription factor or histone modification — is enriched, based on sequencing reads from chromatin immunoprecipitation experiments. It converts raw sequencing data into a set of high-confidence binding or modification sites across the genome, enabling downstream analysis of gene regulation, chromatin state, and epigenetic mechanisms.
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Sources
- Zhang, Y., Liu, T., Meyer, C. A., Eeckhoute, J., Johnson, D. S., Bernstein, B. E., Nusbaum, C., Myers, R. M., Brown, M., Li, W., & Liu, X. S. (2008). Model-based analysis of ChIP-seq (MACS). Genome Biology, 9(9), R137. DOI: 10.1186/gb-2008-9-9-r137 ↗
- Landt, S. G., Marinov, G. K., Kundaje, A., Kheradpour, P., Pauli, F., Batzoglou, S., Bernstein, B. E., Bickel, P., Brown, J. B., Cayting, P., Chen, Y., DeSalvo, G., Epstein, C., Fisher-Aylor, K. I., Euskirchen, G., Gerstein, M., Gertz, J., Hartemink, A. J., Hoffman, M. M., ... Snyder, M. (2012). ChIP-seq guidelines and practices of the ENCODE and modENCODE consortia. Genome Research, 22(9), 1813–1831. DOI: 10.1101/gr.136184.111 ↗
Related methods
Referenced by
Bayesian ChIP-seq peak callingDifferential ChIP-seq peak callingDifferential Epigenome-Wide Association StudyEpigenome-wide association studyMachine learning-assisted ChIP-seq peak callingMulti-omics single-cell RNA-seq analysisRNA-seq Differential ExpressionSequence AlignmentSingle-cell ChIP-seq peak callingTime-series ChIP-seq peak calling