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| Tidsrækkeanalyse af enkeltcelle-RNA-sekventering× | Gen-sætsanrigelsesanalyse (GSEA)× | |
|---|---|---|
| Fagområde | Bioinformatik | Bioinformatik |
| Familie | Process / pipeline | Process / pipeline |
| Oprindelsesår≠ | 2014-2018 (pseudotime and RNA velocity frameworks) | 2005 (seminal PNAS paper; predecessor concept in Mootha et al. 2003) |
| Ophavsperson≠ | Trapnell et al. (pseudotime/Monocle); La Manno et al. (RNA velocity) | Aravind Subramanian, Pablo Tamayo, Vamsi K. Mootha, Jill P. Mesirov, Todd R. Golub, Eric S. Lander et al. (Broad Institute) |
| Type≠ | Computational bioinformatics pipeline | Functional genomics / enrichment analysis |
| Oprindelig kilde≠ | Trapnell, C., Cacchiarelli, D., Grimsby, J., Pokharel, P., Li, S., Morse, M., Lennon, N. J., Livak, K. J., Mikkelsen, T. S., & Rinn, J. L. (2014). The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nature Biotechnology, 32(4), 381-386. DOI ↗ | Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550. DOI ↗ |
| Aliasser | scRNA-seq time course analysis, longitudinal scRNA-seq, temporal single-cell transcriptomics, dynamic single-cell gene expression analysis | GSEA, gene-set analysis, functional enrichment analysis, pathway-level enrichment |
| Relaterede≠ | 6 | 5 |
| Resumé≠ | Time-series single-cell RNA-seq analysis captures gene expression across multiple time points at single-cell resolution to reveal how cell populations emerge, transition, and diverge during dynamic biological processes such as development, differentiation, or disease progression. By combining pseudotime ordering, RNA velocity, and differential dynamics testing, researchers reconstruct the temporal trajectory of individual cells and identify the gene regulatory changes that drive biological transitions. | Gene Set Enrichment Analysis (GSEA) is a computational method that determines whether a predefined set of genes — representing a biological pathway, process, or function — shows statistically significant, coordinated differences between two biological conditions. Unlike simple fold-change filtering, GSEA operates on all measured genes ranked by a correlation metric, detecting subtle but consistent shifts across an entire pathway even when no single gene passes a significance threshold. |
| ScholarGateDatasæt ↗ |
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