方法证据记录
MTT/MTS Assay
The MTT assay, introduced by Tatsuro Mosmann in 1983, is a colorimetric method for quantifying cell viability and proliferation by measuring mitochondrial metabolic activity. The method detects the conversion of the water-soluble tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by active mitochondria, producing an insoluble purple formazan precipitate proportional to the number of viable cells. The related MTS assay, which does not require solubilization, offers improved kinetics and is now widely adopted in both academic research and pharmaceutical development.
源记录
引文逐字复制自方法源记录。这些引文不代表任何层级的验证。
MTT/MTS Cell Viability and Proliferation Assay
分类方法记录 · process-pipeline / biomaterials
- Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63. · DOI 10.1016/0022-1759(83)90303-4
- Slade, P. G. (1999). MTS tetrazolium compound (abstract). Methods in Cell Biology, 63, 65-72. · URL
- Riss, T. L., Moravec, R. A., Niles, A. L., et al. (2004). Cell viability assays. In Assay Guidance Manual. Eli Lilly & Company and the National Center for Advancing Translational Sciences. · URL
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