方法证据记录
Live/Dead Assay
The Live/Dead assay is a fluorescence-based method for simultaneously identifying live and dead cells using two complementary dyes. The assay combines calcein-AM (or SYTO fluorophores), which generates bright green fluorescence in living cells with intact esterase activity, with propidium iodide (PI), which produces red fluorescence in dead cells with compromised membrane integrity. Commercially developed by Molecular Probes and now part of Thermo Fisher's portfolio, the Live/Dead kit is widely used to evaluate cell viability on biomaterial scaffolds, in tissue constructs, and following drug or toxin exposure.
源记录
引文逐字复制自方法源记录。这些引文不代表任何层级的验证。
Live/Dead Cell Viability Fluorescence Assay
分类方法记录 · process-pipeline / biomaterials
- Molecular Probes (2004). LIVE/DEAD Viability/Cytotoxicity Kit user guide. Invitrogen Corporation. · URL
- Niles, A. L., Moravec, R. A., & Riss, T. L. (2009). In vitro viability and cytotoxicity testing and same-well multiplexing assays for adherent cells. Current Chemical Genomics, 3, 33-43. · URL
- Riss, T. L., Moravec, R. A., Niles, A. L., et al. (2011). Cell viability assays. In Assay Guidance Manual (3rd ed.). Eli Lilly & Company and the National Center for Advancing Translational Sciences. · URL
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