方法对比

	表面等离激元共振	圆二色性	SERS
领域	光谱学	光谱学	光谱学
方法族	Process / pipeline	Process / pipeline	Process / pipeline
起源年份	1971	1969	1974
提出者	Erich Kretschmann	Jean-Claude Fasman	Martin Fleischmann
类型	Optical technique	Spectroscopic method	Vibrational spectroscopy technique
开创性文献	Kretschmann, E. (1971). Determination of optical constants of metals by excitation of surface plasmons. Zeitschrift für Physik, 241(4), 313-324.	Greenfield, N. J., & Fasman, G. D. (1969). Computed circular dichroism spectra for protein secondary structures. Biochemistry, 8(10), 4108-4116.	Fleischmann, M., Hendra, P. J., & McQuillan, A. J. (1974). Raman spectra of pyridine adsorbed at a silver electrode. Chemical Physics Letters, 26(2), 163-166.
别名	SPR, surface plasmon, SPR biosensing	CD spectroscopy, circular dichroism, CD analysis	Surface-enhanced Raman scattering, SERS spectroscopy
相关	3	3	3
摘要	Surface Plasmon Resonance (SPR) is a real-time, label-free technique for detecting and monitoring biomolecular interactions at a sensor surface by measuring changes in the refractive index caused by ligand binding. Developed by Kretschmann in 1971 and applied to biosensing by Liedberg, Nylander, and Lundström in 1983, SPR is now a gold standard for measuring binding kinetics (association and dissociation rates) and equilibrium binding constants in protein interactions, antibody-antigen recognition, and drug discovery.	Circular Dichroism (CD) spectroscopy measures the differential absorption of left- and right-circularly polarized light by optically active molecules, particularly proteins and nucleic acids. Introduced by Greenfield and Fasman in 1969, CD is a rapid, non-destructive technique for characterizing secondary structure (alpha-helix, beta-sheet), monitoring protein folding transitions, and assessing conformational changes in response to pH, temperature, or ligand binding.	Surface-Enhanced Raman Spectroscopy (SERS) amplifies weak Raman signals by many orders of magnitude when analyte molecules are adsorbed on specially prepared metal (typically silver or gold) nanostructured surfaces. Discovered by Fleischmann, Hendra, and McQuillan in 1974, SERS enables detection of vibrational signatures of single molecules and ultra-trace contaminants, revolutionizing analytical chemistry and forensics.