方法对比

	划痕伤口测定法	MTT/MTS 测定法
领域	生物材料	生物材料
方法族	Process / pipeline	Process / pipeline
起源年份	2007	1983
提出者	Liang, Park, and Guan	Tatsuro Mosmann
类型	Migration assessment	Colorimetric assay
开创性文献	Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333.	Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63.
别名	wound healing assay, gap closure assay, migration assay	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, tetrazolium assay, mitochondrial activity assay
相关	4	4
摘要	The scratch wound assay (also called the wound healing assay or gap closure assay) is a simple, cost-effective method for measuring cell migration in vitro. Developed and standardized by Liang, Park, and Guan in 2007, the assay involves creating a defined gap (wound) in a monolayer of confluent cells using a pipette tip or specialized tool, then monitoring the rate at which cells migrate into the gap over hours to days. The scratch wound assay is widely used to evaluate the effects of growth factors, inhibitory compounds, and biomaterial extracts on cell motility and wound healing potential.	The MTT assay, introduced by Tatsuro Mosmann in 1983, is a colorimetric method for quantifying cell viability and proliferation by measuring mitochondrial metabolic activity. The method detects the conversion of the water-soluble tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by active mitochondria, producing an insoluble purple formazan precipitate proportional to the number of viable cells. The related MTS assay, which does not require solubilization, offers improved kinetics and is now widely adopted in both academic research and pharmaceutical development.