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| RDE Koutecky-Levich× | 表面等离激元共振× | |
|---|---|---|
| 领域 | 光谱学 | 光谱学 |
| 方法族 | Process / pipeline | Process / pipeline |
| 起源年份≠ | 1962 | 1971 |
| 提出者≠ | Veniamin Levich | Erich Kretschmann |
| 类型≠ | Electrochemical technique | Optical technique |
| 开创性文献≠ | Levich, V. G. (1962). Physicochemical Hydrodynamics. Prentice Hall. link ↗ | Kretschmann, E. (1971). Determination of optical constants of metals by excitation of surface plasmons. Zeitschrift für Physik, 241(4), 313-324. link ↗ |
| 别名 | RDE, rotating disk electrode, Koutecky-Levich | SPR, surface plasmon, SPR biosensing |
| 相关 | 3 | 3 |
| 摘要≠ | Rotating Disk Electrode (RDE) electrochemistry combined with Koutecky-Levich analysis is a powerful electrochemical technique that decouples diffusion-limited and kinetically limited electron-transfer processes. Developed by Levich in the 1960s, RDE enables determination of heterogeneous electron-transfer rate constants and mechanistic information by rotating an electrode to control mass transport. | Surface Plasmon Resonance (SPR) is a real-time, label-free technique for detecting and monitoring biomolecular interactions at a sensor surface by measuring changes in the refractive index caused by ligand binding. Developed by Kretschmann in 1971 and applied to biosensing by Liedberg, Nylander, and Lundström in 1983, SPR is now a gold standard for measuring binding kinetics (association and dissociation rates) and equilibrium binding constants in protein interactions, antibody-antigen recognition, and drug discovery. |
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