方法对比
并排查看您选择的方法；存在差异的行会高亮显示。
| 活/死细胞检测试剂盒× | 划痕伤口测定法× | |
|---|---|---|
| 领域 | 生物材料 | 生物材料 |
| 方法族 | Process / pipeline | Process / pipeline |
| 起源年份≠ | 2000 | 2007 |
| 提出者≠ | Invitrogen/Molecular Probes | Liang, Park, and Guan |
| 类型≠ | Dual-dye viability assay | Migration assessment |
| 开创性文献≠ | Molecular Probes (2004). LIVE/DEAD Viability/Cytotoxicity Kit user guide. Invitrogen Corporation. link ↗ | Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333. DOI ↗ |
| 别名 | calcein-AM/propidium iodide, SYTO/PI staining, fluorescent viability stain | wound healing assay, gap closure assay, migration assay |
| 相关 | 4 | 4 |
| 摘要≠ | The Live/Dead assay is a fluorescence-based method for simultaneously identifying live and dead cells using two complementary dyes. The assay combines calcein-AM (or SYTO fluorophores), which generates bright green fluorescence in living cells with intact esterase activity, with propidium iodide (PI), which produces red fluorescence in dead cells with compromised membrane integrity. Commercially developed by Molecular Probes and now part of Thermo Fisher's portfolio, the Live/Dead kit is widely used to evaluate cell viability on biomaterial scaffolds, in tissue constructs, and following drug or toxin exposure. | The scratch wound assay (also called the wound healing assay or gap closure assay) is a simple, cost-effective method for measuring cell migration in vitro. Developed and standardized by Liang, Park, and Guan in 2007, the assay involves creating a defined gap (wound) in a monolayer of confluent cells using a pipette tip or specialized tool, then monitoring the rate at which cells migrate into the gap over hours to days. The scratch wound assay is widely used to evaluate the effects of growth factors, inhibitory compounds, and biomaterial extracts on cell motility and wound healing potential. |
| ScholarGate数据集 ↗ |
|
|