方法对比
并排查看您选择的方法；存在差异的行会高亮显示。
| 等温滴定量热法× | 圆二色性× | |
|---|---|---|
| 领域 | 光谱学 | 光谱学 |
| 方法族 | Process / pipeline | Process / pipeline |
| 起源年份≠ | 1989 | 1969 |
| 提出者≠ | Terrence Wiseman | Jean-Claude Fasman |
| 类型≠ | Biophysical technique | Spectroscopic method |
| 开创性文献≠ | Wiseman, T., Williston, S., Brandts, J. F., & Lin, L. N. (1989). Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Analytical Biochemistry, 179(1), 131-137. DOI ↗ | Greenfield, N. J., & Fasman, G. D. (1969). Computed circular dichroism spectra for protein secondary structures. Biochemistry, 8(10), 4108-4116. DOI ↗ |
| 别名 | ITC, isothermal calorimetry, microcalorimetry | CD spectroscopy, circular dichroism, CD analysis |
| 相关 | 3 | 3 |
| 摘要≠ | Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that measures heat released or absorbed during biomolecular binding events at constant temperature. Developed by Wiseman and colleagues in 1989, ITC directly determines binding affinity (Kd), enthalpy (ΔH), and entropy (ΔS) in a single experiment, making it one of the most comprehensive methods for characterizing molecular interactions without requiring labels or immobilization. | Circular Dichroism (CD) spectroscopy measures the differential absorption of left- and right-circularly polarized light by optically active molecules, particularly proteins and nucleic acids. Introduced by Greenfield and Fasman in 1969, CD is a rapid, non-destructive technique for characterizing secondary structure (alpha-helix, beta-sheet), monitoring protein folding transitions, and assessing conformational changes in response to pH, temperature, or ligand binding. |
| ScholarGate数据集 ↗ |
|
|