方法对比
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| 血凝抑制试验× | 酶联免疫吸附测定 (ELISA)× | |
|---|---|---|
| 领域 | 兽医科学 | 兽医科学 |
| 方法族 | Process / pipeline | Process / pipeline |
| 起源年份≠ | 1942 | 1971 |
| 提出者≠ | George K. Hirst | Eva Engvall and Peter Perlmann; independent parallel development by Anton Schuurs and Bauke van Weemen |
| 类型≠ | Serological diagnostic assay | Quantitative immunoassay |
| 开创性文献≠ | Hirst, G. K. (1942). The quantitative determination of influenza virus and antibodies by means of red cell agglutination. Journal of Experimental Medicine, 75(1), 49–64. DOI ↗ | Engvall, E., & Perlmann, P. (1971). Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry, 8(9), 871–874. DOI ↗ |
| 别名 | HI assay, HAI test, haemagglutination inhibition test, HI test | enzyme immunoassay, EIA, solid-phase enzyme immunoassay, ELISA test |
| 相关≠ | 1 | 2 |
| 摘要≠ | The Hemagglutination Inhibition (HI) Assay is a classical serological test used to detect and quantify antibodies against hemagglutinating viruses — most notably influenza and Newcastle disease virus — in animal and human serum. Widely employed in veterinary diagnostics, vaccine efficacy evaluation, and epidemiological surveillance, it relies on the principle that specific antibodies in serum will block a known quantity of virus from agglutinating red blood cells. | ELISA is a plate-based immunoassay technique that detects and quantifies proteins, antibodies, antigens, hormones, and other analytes in biological samples. Widely used in veterinary science, medicine, and food safety, it exploits the specificity of antibody–antigen binding coupled to an enzyme-driven colorimetric signal to deliver sensitive, reproducible measurements across large sample batches. |
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