Process / pipelineBioinformatics / omics
单细胞序列比对 — scRNA-seq 读段比对
单细胞序列比对是计算步骤，它将单细胞 RNA 测序实验产生的数百万个短测序读段映射回参考基因组或转录组。与批量 RNA 测序比对不同，每个读段都带有一个细胞条形码和一个唯一分子标识符 (UMI)，它们共同识别起始细胞和单个 RNA 分子。准确的比对和条形码解复用是构建驱动所有下游单细胞分析的细胞-基因计数矩阵的先决条件。
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来源
- Dobin, A., Davis, C. A., Schlesinger, F., Drenkow, J., Zaleski, C., Jha, S., Batut, P., Chaisson, M., & Gingeras, T. R. (2013). STAR: ultrafast universal RNA-seq aligner. Bioinformatics, 29(1), 15–21. DOI: 10.1093/bioinformatics/bts635 ↗
- Smith, T., Heger, A., & Sudbery, I. (2017). UMI-tools: modeling sequencing errors in Unique Molecular Identifiers to improve quantification accuracy. Genome Research, 27(3), 491–499. DOI: 10.1101/gr.209601.116 ↗
如何引用本页
ScholarGate. (2026, June 3). Single-cell RNA-seq Sequence Alignment. ScholarGate. https://scholargate.app/zh/bioinformatics/single-cell-sequence-alignment
Which method?
Set this method beside its closest kin and read them side by side — the library lays the books on the table; the choice is yours.
- RNA-seq差异表达生物信息学↔ compare