So sánh phương pháp

	Căn chỉnh trình tự	Variant Calling
Lĩnh vực	Tin sinh học	Tin sinh học
Họ	Process / pipeline	Process / pipeline
Năm ra đời	1970 (global alignment); 1981 (local alignment)	2009–2010 (modern high-throughput era)
Người khởi xướng	Saul B. Needleman & Christian D. Wunsch (global); Temple F. Smith & Michael S. Waterman (local)	Li et al. (SAMtools/bcftools, 2009); McKenna et al. (GATK, 2010)
Loại	Computational sequence analysis technique	Computational genomics pipeline
Công trình gốc	Needleman, S. B., & Wunsch, C. D. (1970). A general method applicable to the search for similarities in the amino acid sequence of two proteins. Journal of Molecular Biology, 48(3), 443–453.	McKenna, A., Hanna, M., Banks, E., Sivachenko, A., Cibulskis, K., Kernytsky, A., ... & DePristo, M. A. (2010). The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Research, 20(9), 1297–1303.
Tên gọi khác	pairwise alignment, multiple sequence alignment, MSA, sequence comparison	SNP calling, genotyping from sequencing, mutation detection, variant detection
Liên quan	6	6
Tóm tắt	Sequence alignment is a foundational bioinformatics technique that arranges two or more DNA, RNA, or protein sequences to reveal regions of similarity, infer evolutionary relationships, identify functional domains, and map sequencing reads to reference genomes. It underpins virtually every downstream genomic analysis, from variant calling and gene expression quantification to phylogenetics and structural annotation.	Variant calling is the computational process of identifying positions in a sequenced genome that differ from a reference sequence — including single nucleotide polymorphisms (SNPs), small insertions and deletions (indels), and structural variants. It transforms aligned sequencing reads into an interpretable catalogue of genetic differences, forming the foundation for population genetics, disease-gene discovery, and clinical genomics applications.