So sánh phương pháp

	Phân tích Hi-C	Phân tích ATAC-seq	Vận tốc RNA (RNA Velocity)
Lĩnh vực	Di truyền học	Di truyền học	Di truyền học
Họ	Process / pipeline	Process / pipeline	Process / pipeline
Năm ra đời	2009	2013	2018
Người khởi xướng	Erez Lieberman-Aiden & Job Dekker	Jason Buenrostro, Paul Giresi & William Greenleaf	Gioele La Manno & Pavel Soldatov
Loại	Chromatin interaction method	Chromatin profiling method	Trajectory inference method
Công trình gốc	Lieberman-Aiden, E., van Berkum, N. L., Williams, L., Imakaev, M., Ragoczy, T., Telling, A., & Dekker, J. (2009). Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science, 326(5950), 289–293.	Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y., & Greenleaf, W. J. (2013). Transposition of native chromatin for fast and sensitive epigenomic profiling of cell populations and tissues. Nature Methods, 10(12), 1213–1218.	La Manno, G., Soldatov, R., Zeisel, A., Braun, E., Hochgerner, H., Petukhov, V., & Merad, M. (2018). RNA velocity of single cells. Nature, 560(7737), 494–498.
Tên gọi khác	Chromosome conformation capture, 3D genome, Chromatin contact mapping	Chromatin accessibility, Open chromatin, Accessible chromatin analysis	Velocity analysis, Transcriptomic velocity, Cell fate prediction
Liên quan	2	2	2
Tóm tắt	Hi-C (High-Chromosome Conformation Capture) is a technique and associated computational methods for mapping the 3D architecture of the genome within cells. Developed by Lieberman-Aiden and Dekker in 2009, Hi-C identifies physical interactions between genomic regions that may be distant in linear sequence but spatially proximal in 3D nuclear space. Hi-C analysis has revealed fundamental principles of genome organization, including the existence of topologically associating domains (TADs), and provides insights into how 3D structure regulates gene expression and DNA replication.	ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a method for profiling the landscape of chromatin accessibility genome-wide. Developed by Buenrostro and colleagues in 2013, ATAC-seq uses hyperactive transposase to tag open, accessible chromatin regions, enabling rapid and sensitive identification of regulatory DNA elements. ATAC-seq has become a standard technique for characterizing gene regulatory landscapes, discovering cell-type-specific regulatory elements, and inferring gene regulatory networks.	RNA velocity is a computational method that infers the future developmental state of individual cells from single-cell RNA-sequencing data. Developed by La Manno and colleagues in 2018, RNA velocity analysis measures the direction and pace of cell state transitions by analyzing the ratio of unspliced to spliced mRNA transcripts within individual cells. This enables prediction of cell trajectories and differentiation pathways without requiring temporal sampling or manipulation, providing unique insights into cell fate decisions during development and disease.