เปรียบเทียบวิธี

	การสั่นพ้องของพลาสมอนพื้นผิว	Circular Dichroism
สาขาวิชา	สเปกโทรสโกปี	สเปกโทรสโกปี
ตระกูล	Process / pipeline	Process / pipeline
ปีกำเนิด	1971	1969
ผู้ริเริ่ม	Erich Kretschmann	Jean-Claude Fasman
ประเภท	Optical technique	Spectroscopic method
แหล่งต้นตำรับ	Kretschmann, E. (1971). Determination of optical constants of metals by excitation of surface plasmons. Zeitschrift für Physik, 241(4), 313-324.	Greenfield, N. J., & Fasman, G. D. (1969). Computed circular dichroism spectra for protein secondary structures. Biochemistry, 8(10), 4108-4116.
ชื่อเรียกอื่น	SPR, surface plasmon, SPR biosensing	CD spectroscopy, circular dichroism, CD analysis
ที่เกี่ยวข้อง	3	3
สรุป	Surface Plasmon Resonance (SPR) is a real-time, label-free technique for detecting and monitoring biomolecular interactions at a sensor surface by measuring changes in the refractive index caused by ligand binding. Developed by Kretschmann in 1971 and applied to biosensing by Liedberg, Nylander, and Lundström in 1983, SPR is now a gold standard for measuring binding kinetics (association and dissociation rates) and equilibrium binding constants in protein interactions, antibody-antigen recognition, and drug discovery.	Circular Dichroism (CD) spectroscopy measures the differential absorption of left- and right-circularly polarized light by optically active molecules, particularly proteins and nucleic acids. Introduced by Greenfield and Fasman in 1969, CD is a rapid, non-destructive technique for characterizing secondary structure (alpha-helix, beta-sheet), monitoring protein folding transitions, and assessing conformational changes in response to pH, temperature, or ligand binding.