เปรียบเทียบวิธี

	eDNA Metabarcoding	Distance Sampling
สาขาวิชา	นิเวศวิทยา	นิเวศวิทยา
ตระกูล	Process / pipeline	Process / pipeline
ปีกำเนิด	2012	1993
ผู้ริเริ่ม	Pierre Taberlet	Stephen Buckland
ประเภท	species detection and community assessment	population abundance estimation
แหล่งต้นตำรับ	Taberlet, P., Coissac, E., Hajibabaei, M., & Rieseberg, L. H. (2012). Environmental DNA. Molecular Ecology, 21(8), 1789-1793.	Buckland, S. T., Anderson, D. R., Burnham, K. P., Laake, J. L., Borchers, D. L., & Thomas, L. (1993). Distance Sampling: Estimating Abundance of Biological Populations. Chapman and Hall, London.
ชื่อเรียกอื่น	eDNA, metabarcoding, DNA metabarcoding, genetic monitoring	line transect, point transect, distance estimation, detection probability
ที่เกี่ยวข้อง	4	4
สรุป	Environmental DNA (eDNA) metabarcoding detects and identifies species present in environmental samples (water, soil, air) by sequencing short DNA fragments released by organisms. Developed by Taberlet and colleagues (2012), this approach has revolutionized biodiversity monitoring: species can be surveyed without capture, observation, or complex sampling designs. Metabarcoding sequences millions of DNA fragments, identifies reads taxonomically, and assigns them to species. The method is non-invasive, rapid, and cost-effective, enabling large-scale biodiversity surveys and early detection of cryptic or rare species.	Distance sampling is a statistical method for estimating population abundance from data on distances between observers and detected individuals. Developed by Buckland and colleagues (1993) and formalized in the software Distance, this approach accounts for imperfect detection: animals far from an observer are less likely to be detected. By modeling the detection function (probability of detecting an animal at various distances), distance sampling produces unbiased estimates of abundance and density even when detection is incomplete.