เปรียบเทียบวิธี

	Circular Dichroism	การสั่นพ้องของพลาสมอนพื้นผิว
สาขาวิชา	สเปกโทรสโกปี	สเปกโทรสโกปี
ตระกูล	Process / pipeline	Process / pipeline
ปีกำเนิด	1969	1971
ผู้ริเริ่ม	Jean-Claude Fasman	Erich Kretschmann
ประเภท	Spectroscopic method	Optical technique
แหล่งต้นตำรับ	Greenfield, N. J., & Fasman, G. D. (1969). Computed circular dichroism spectra for protein secondary structures. Biochemistry, 8(10), 4108-4116.	Kretschmann, E. (1971). Determination of optical constants of metals by excitation of surface plasmons. Zeitschrift für Physik, 241(4), 313-324.
ชื่อเรียกอื่น	CD spectroscopy, circular dichroism, CD analysis	SPR, surface plasmon, SPR biosensing
ที่เกี่ยวข้อง	3	3
สรุป	Circular Dichroism (CD) spectroscopy measures the differential absorption of left- and right-circularly polarized light by optically active molecules, particularly proteins and nucleic acids. Introduced by Greenfield and Fasman in 1969, CD is a rapid, non-destructive technique for characterizing secondary structure (alpha-helix, beta-sheet), monitoring protein folding transitions, and assessing conformational changes in response to pH, temperature, or ligand binding.	Surface Plasmon Resonance (SPR) is a real-time, label-free technique for detecting and monitoring biomolecular interactions at a sensor surface by measuring changes in the refractive index caused by ligand binding. Developed by Kretschmann in 1971 and applied to biosensing by Liedberg, Nylander, and Lundström in 1983, SPR is now a gold standard for measuring binding kinetics (association and dissociation rates) and equilibrium binding constants in protein interactions, antibody-antigen recognition, and drug discovery.