Linganisha mbinu

	Uchanganuzi wa usemi wa RNA-seq wa vipindi vya muda	Uchanganuzi wa RNA-seq wa seli moja
Nyanja	Bioinformatiki	Bioinformatiki
Familia	Process / pipeline	Process / pipeline
Mwaka wa asili	2006–2018 (principal methods established)	2009 (first scRNA-seq by Tang et al.); widely adopted 2015–2016
Mwanzilishi	Conesa et al. (maSigPro, 2006); extended by Fischer et al. (ImpulseDE2, 2018) and others	Azim Surani, Barbara Treutlein, and the Regev/McCarroll groups (foundational droplet-based methods ~2015)
Aina	Computational genomics pipeline	High-throughput single-cell transcriptomic profiling pipeline
Chanzo asilia	Conesa, A., Nueda, M. J., Ferrer, A., & Talon, M. (2006). maSigPro: a method to identify significantly differential expression profiles in time-course microarray experiments. Bioinformatics, 22(9), 1096–1102.	Satija, R., Farrell, J. A., Gennert, D., Schier, A. F., & Regev, A. (2015). Spatial reconstruction of single-cell gene expression data. Nature Biotechnology, 33(5), 495–502.
Majina mbadala	longitudinal RNA-seq DE analysis, temporal transcriptomics, time-course RNA-seq, dynamic DE analysis	scRNA-seq, single-cell transcriptomics, scRNAseq analysis, single-cell gene expression profiling
Zinazohusiana	6	5
Muhtasari	Time-series RNA-seq differential expression analysis identifies genes whose expression levels change systematically across ordered time points — such as during development, disease progression, or response to a treatment. Unlike two-condition DE analysis, it explicitly models the temporal structure of the data, capturing dynamic gene expression trajectories rather than a single snapshot contrast. Tools such as maSigPro, ImpulseDE2, and splineTimeR have been developed specifically for this design.	Single-cell RNA sequencing (scRNA-seq) analysis characterises gene expression at the resolution of individual cells, enabling discovery of cell types, states, and transitions that are invisible in bulk transcriptomics. Starting from raw sequencing reads, the workflow produces a cell-by-gene count matrix and proceeds through quality control, normalisation, dimensionality reduction, unsupervised clustering, cell-type annotation, and a range of downstream analyses such as trajectory inference and differential expression between cell populations.