Linganisha mbinu

	Uchambuzi wa Anuwai ya Mikrobiomu kwa Kutumia Mbinu za Multi-omics	Uchanganuzi wa Uboreshaji wa Njia
Nyanja	Bioinformatiki	Bioinformatiki
Familia	Process / pipeline	Process / pipeline
Mwaka wa asili	2010s–present	2003–2005
Mwanzilishi	Developed collectively; key frameworks by Le Cao et al. (mixOmics, 2017) and Argelaguet et al. (MOFA, 2018)	Mootha et al. (2003); systematised by Subramanian et al. (2005)
Aina	Integrative computational pipeline	Statistical functional annotation method
Chanzo asilia	Rohart, F., Gautier, B., Singh, A., & Le Cao, K.-A. (2017). mixOmics: An R package for 'omics feature selection and multiple data integration. PLOS Computational Biology, 13(11), e1005752.	Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550.
Majina mbadala	multi-omics microbiome profiling, integrated microbiome omics, multi-modal microbiome analysis, microbiome multi-omics integration	PEA, overrepresentation analysis, ORA, functional enrichment analysis
Zinazohusiana	5	6
Muhtasari	Multi-omics microbiome diversity analysis integrates two or more omic data layers — such as metagenomics, metatranscriptomics, metabolomics, and metaproteomics — to characterise both the composition and functional activity of microbial communities. By linking taxonomic diversity metrics with molecular phenotype data, the approach uncovers how community structure translates into ecological and host-relevant functions that no single omic layer can reveal alone.	Pathway enrichment analysis (PEA) is a statistical approach that takes a list of genes or proteins of interest — typically derived from a differential expression or proteomics experiment — and identifies which pre-defined biological pathways or functional gene sets are represented more often than expected by chance. By mapping individual molecular changes onto curated pathway knowledge bases such as KEGG, Gene Ontology, or Reactome, PEA translates long gene lists into interpretable biological processes, making it a central tool in the post-analysis of high-throughput omics experiments.