Linganisha mbinu

	Upigaji Picha wa Cytometry ya Misa	Ultrasoundi wa Kazi
Nyanja	Upigaji Picha za Kitiba	Upigaji Picha za Kitiba
Familia	Process / pipeline	Process / pipeline
Mwaka wa asili	2014	2011
Mwanzilishi	Bernd Bodenmiller	Mickael Tanter
Aina	Multiplexed single-cell imaging by mass spectrometry	High-framerate doppler imaging for hemodynamics
Chanzo asilia	Giesen, C., Wang, H. A., Schapiro, D., et al. (2014). Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nature Methods, 11(4), 417-422.	Macé, E., Montaldo, G., Trenholm, S., et al. (2011). Functional ultrasound imaging of the brain. Nature Methods, 8(8), 662-664.
Majina mbadala	IMC, mass cytometry, multiplex ion beam imaging, MIBI	fUS, doppler ultrasound, ultrafast ultrasound
Zinazohusiana	5	5
Muhtasari	Imaging Mass Cytometry (IMC) is a multiplexed proteomics technique that maps the subcellular localization of up to 40-50 proteins in tissue sections simultaneously using mass spectrometry detection. Developed by Bodenmiller and colleagues in 2014, IMC combines the single-cell imaging power of immunofluorescence with the multiplexing capacity of mass cytometry, enabling comprehensive analysis of cell types, states, and spatial interactions within tissue microenvironments. IMC has emerged as a powerful tool in immuno-oncology, immunobiology, and tissue biology for dissecting cellular heterogeneity and spatial organization.	Functional Ultrasound (fUS) is a high-framerate Doppler ultrasound technique that dynamically maps blood flow and hemodynamic changes in vivo with millisecond temporal resolution. Pioneered by Tanter, Macé, and colleagues in the 2010s, fUS enables real-time imaging of microvascular perfusion in the brain and other organs. By combining ultrafast acquisition (1000-5000 frames per second) with Doppler processing, fUS reveals functional activity (hemodynamic changes during stimulation or behavior) and vascular networks with unprecedented spatiotemporal detail.