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	Multi-omik metabolomanalys – Integrering av metaboliter med andra omikskikt	Genuppsättningsanrikningsanalys (GSEA)
Ämnesområde	Bioinformatik	Bioinformatik
Familj	Process / pipeline	Process / pipeline
Ursprungsår	2000s–2010s (metabolomics ~2000; multi-omics integration ~2010s)	2005 (seminal PNAS paper; predecessor concept in Mootha et al. 2003)
Upphovsperson	Pioneered collectively; key early integrative frameworks by Nicholson & Lindon (metabolomics) and Hasin, Seldin & Lusis (multi-omics disease mapping)	Aravind Subramanian, Pablo Tamayo, Vamsi K. Mootha, Jill P. Mesirov, Todd R. Golub, Eric S. Lander et al. (Broad Institute)
Typ	Integrative computational pipeline	Functional genomics / enrichment analysis
Ursprungskälla	Subramanian, I., Verma, S., Kumar, S., Jere, A., & Anamika, K. (2020). Multi-omics data integration, interpretation, and its application. Bioinformatics and Biology Insights, 14, 1177932219899051.	Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550.
Alias	metabolomics multi-omics integration, integrated metabolomics, multi-omics metabolite profiling, metabolome-centric multi-omics	GSEA, gene-set analysis, functional enrichment analysis, pathway-level enrichment
Närliggande	5	5
Sammanfattning	Multi-omics metabolomics analysis integrates metabolite profiling data — derived from mass spectrometry or NMR spectroscopy — with genomic, transcriptomic, and/or proteomic datasets to build a system-level view of biological phenotypes. By anchoring integration on the metabolome, which reflects the downstream functional output of gene expression and protein activity, this approach connects upstream molecular variation to observable biochemical states, enabling richer mechanistic insight than any single omics layer alone.	Gene Set Enrichment Analysis (GSEA) is a computational method that determines whether a predefined set of genes — representing a biological pathway, process, or function — shows statistically significant, coordinated differences between two biological conditions. Unlike simple fold-change filtering, GSEA operates on all measured genes ranked by a correlation metric, detecting subtle but consistent shifts across an entire pathway even when no single gene passes a significance threshold.