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| Elektrofiziologija metodom jedinstvenog spoja (patch-clamp)× | Šildova analiza× | |
|---|---|---|
| Oblast | Farmakologija | Farmakologija |
| Porodica | Process / pipeline | Process / pipeline |
| Godina nastanka≠ | 1976 | 1947 |
| Tvorac≠ | Erwin Neher and Bert Sakmann | Henry Schild |
| Tip≠ | ion channel screening | antagonism quantification |
| Temeljni izvor≠ | Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802. DOI ↗ | Schild, H. O. (1947). pA, a new scale for the measurement of drug antagonism. Journal of Physiology, 106(3), 337-357. DOI ↗ |
| Drugi nazivi≠ | patch clamp, whole-cell recording, ion channel assay | Schild plot, pA2 |
| Srodne | 3 | 3 |
| Sažetak≠ | Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment. | Schild analysis is a quantitative method for characterizing competitive receptor antagonism developed by Henry Schild in 1947. It uses dose-response curves in the presence and absence of antagonist to estimate the antagonist affinity constant (pA2), enabling standardized comparison of antagonist potency across drugs and experimental systems. |
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