Uporedite metode

	Analiza jednoćelijske RNA-sekvenciranja više omika	RNA-seq Differential Expression
Oblast	Bioinformatika	Bioinformatika
Porodica	Process / pipeline	Process / pipeline
Godina nastanka	2015–2021 (rapid maturation with CITE-seq 2017; Seurat v4 2021)	2008–2010 (RNA-seq DE methodology established)
Tvorac	Pioneered by Rahul Satija (Seurat), Oliver Stegle and John Marioni (MOFA+), and the broader single-cell genomics community	Multiple groups; foundational methods from Anders & Huber (DESeq, 2010), Robinson, McCarthy & Smyth (edgeR, 2010)
Tip	Integrative computational pipeline	Quantitative genomics pipeline
Temeljni izvor	Hao, Y., Hao, S., Andersen-Nissen, E., Mauck, W. M., Zheng, S., Butler, A., Lee, M. J., Wilk, A. J., Darby, C., Zager, M., Hoffman, P., Stoeckius, M., Papalexi, E., Mimitou, E. P., Jain, J., Srivastava, A., Stuart, T., Fleming, L. M., Yeung, B., Rogers, A. J., McElrath, J. M., Blish, C. A., Gottardo, R., Smibert, P., & Satija, R. (2021). Integrated analysis of multimodal single-cell data. Cell, 184(13), 3573–3587.e29.	Love, M. I., Huber, W., & Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology, 15(12), 550.
Drugi nazivi	scMulti-omics, single-cell multi-omics, multimodal single-cell analysis, paired single-cell omics	RNA-seq DE analysis, transcriptomic differential expression, bulk RNA-seq DE, DEA
Srodne	6	6
Sažetak	Multi-omics single-cell RNA-seq analysis integrates two or more molecular layers — such as gene expression (scRNA-seq), chromatin accessibility (scATAC-seq), or surface protein abundance (CITE-seq) — measured simultaneously or co-profiled in the same individual cells. By aligning these modalities in a shared low-dimensional space, researchers gain a mechanistically richer picture of cell identity, regulatory state, and phenotype than any single assay can provide.	RNA-seq differential expression (DE) analysis identifies genes whose transcript abundance differs significantly between two or more biological conditions — for example, treated versus control, or diseased versus healthy tissue. Starting from raw sequencing reads, the pipeline moves through alignment, count-based normalization, statistical modeling of count dispersion, hypothesis testing, and multiple-testing correction to produce a ranked list of differentially expressed genes accompanied by fold-change estimates and adjusted p-values.