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| Analiza protočne citometrije× | Elektrofiziologija metodom jedinstvenog spoja (patch-clamp)× | |
|---|---|---|
| Oblast | Farmakologija | Farmakologija |
| Porodica | Process / pipeline | Process / pipeline |
| Godina nastanka | 1976 | 1976 |
| Tvorac≠ | Leonard Herzenberg | Erwin Neher and Bert Sakmann |
| Tip≠ | cell analysis and sorting | ion channel screening |
| Temeljni izvor≠ | Herzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M., & Herzenberg, L. A. (2002). The history and future of the fluorescence-activated cell sorter and flow cytometry: a view from Stanford. Clinical Chemistry, 48(10), 1819-1827. DOI ↗ | Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802. DOI ↗ |
| Drugi nazivi | FACS, fluorescence-activated cell sorting, cell analysis | patch clamp, whole-cell recording, ion channel assay |
| Srodne | 3 | 3 |
| Sažetak≠ | Flow cytometry is a laser-based technology for analyzing and sorting individual cells based on fluorescent markers. Developed by Leonard Herzenberg in the 1970s, flow cytometry enables rapid assessment of cell phenotype, drug effects on cell populations, and therapeutic cell characterization in immunology and hematology. | Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment. |
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