Porovnať metódy

	Transwell test	CAM test
Odbor	Biomateriály	Biomateriály
Rodina	Process / pipeline	Process / pipeline
Rok vzniku	1962	1974
Tvorca	Stephen Boyden	Judah Folkman
Typ	Migration/invasion assay	Developmental biology assay
Pôvodný zdroj	Boyden, S. (1962). The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. Journal of Experimental Medicine, 115(3), 453-466.	Folkman, J. (1974). Tumor angiogenesis: therapeutic implications. New England Journal of Medicine, 285(21), 1182-1186.
Ďalšie názvy	Boyden chamber assay, chemotaxis assay, invasion chamber assay	chick embryo chorioallantoic membrane, angiogenesis assay, CAM angiogenesis model
Príbuzné	4	4
Zhrnutie	The Transwell assay (also called the Boyden chamber assay after its originator Stephen Boyden) is a quantitative method for measuring cell migration and invasion in response to chemical gradients or through matrix barriers. The assay uses a membrane insert with defined pore size suspended in a multi-well plate: cells are placed in the upper chamber, a chemoattractant is placed in the lower chamber, and cells that successfully migrate through the pores accumulate in the lower chamber, where they can be counted or visualized. Variants that coat the insert with matrix proteins (Matrigel, collagen) enable measurement of invasion capacity. The Transwell assay is a gold-standard method in cell biology for evaluating cell motility, tumor metastatic potential, and the effects of growth factors and inhibitory compounds.	The chorioallantoic membrane (CAM) assay is a well-established in vivo model for studying angiogenesis (new blood vessel formation) and evaluating the pro- or anti-angiogenic properties of biomaterials, drugs, and bioactive molecules. Developed by Judah Folkman in the 1970s, the assay uses the highly vascularized CAM of developing chick embryos as a platform for implanting test materials and observing vascular response. The CAM provides a transparent, immunologically naive microenvironment with rapid and reproducible neovascularization, making it ideal for screening angiogenic potential and assessing biomaterial biocompatibility.