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Enzyme-Linked Immunosorbent Assay (ELISA)×Analýza prietokovou cytometriou×Radioimmunoassay (RIA)×
OdborVeterinárne vedyFarmakológiaVeterinárne vedy
RodinaProcess / pipelineProcess / pipelineProcess / pipeline
Rok vzniku197119761959–1960
TvorcaEva Engvall and Peter Perlmann; independent parallel development by Anton Schuurs and Bauke van WeemenLeonard HerzenbergRosalyn Yalow and Solomon Berson
TypQuantitative immunoassaycell analysis and sortingQuantitative immunological assay
Pôvodný zdrojEngvall, E., & Perlmann, P. (1971). Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry, 8(9), 871–874. DOI ↗Herzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M., & Herzenberg, L. A. (2002). The history and future of the fluorescence-activated cell sorter and flow cytometry: a view from Stanford. Clinical Chemistry, 48(10), 1819-1827. DOI ↗Yalow, R. S., & Berson, S. A. (1960). Immunoassay of endogenous plasma insulin in man. Journal of Clinical Investigation, 39(7), 1157–1175. DOI ↗
Ďalšie názvyenzyme immunoassay, EIA, solid-phase enzyme immunoassay, ELISA testFACS, fluorescence-activated cell sorting, cell analysisRIA, radioisotope immunoassay, isotope immunoassay, radioligand assay
Príbuzné231
ZhrnutieELISA is a plate-based immunoassay technique that detects and quantifies proteins, antibodies, antigens, hormones, and other analytes in biological samples. Widely used in veterinary science, medicine, and food safety, it exploits the specificity of antibody–antigen binding coupled to an enzyme-driven colorimetric signal to deliver sensitive, reproducible measurements across large sample batches.Flow cytometry is a laser-based technology for analyzing and sorting individual cells based on fluorescent markers. Developed by Leonard Herzenberg in the 1970s, flow cytometry enables rapid assessment of cell phenotype, drug effects on cell populations, and therapeutic cell characterization in immunology and hematology.Radioimmunoassay (RIA) is a highly sensitive, quantitative laboratory technique that measures the concentration of a specific antigen — such as a hormone, drug, or pathogen-derived protein — in a biological sample by exploiting competitive binding between a radiolabelled antigen and the sample antigen for a limited supply of specific antibody. Developed in the late 1950s, RIA is widely used in veterinary science, endocrinology, pharmacology, and clinical diagnostics.
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ScholarGatePorovnať metódy: Enzyme-Linked Immunosorbent Assay (ELISA) · Flow Cytometry · Radioimmunoassay. Získané 2026-06-19 z https://scholargate.app/sk/compare