Porovnať metódy
Prezrite si vybrané metódy vedľa seba; riadky, ktoré sa líšia, sú zvýraznené.
| Cirkulárna dichroickosť× | SERS× | |
|---|---|---|
| Odbor | Spektroskopia | Spektroskopia |
| Rodina | Process / pipeline | Process / pipeline |
| Rok vzniku≠ | 1969 | 1974 |
| Tvorca≠ | Jean-Claude Fasman | Martin Fleischmann |
| Typ≠ | Spectroscopic method | Vibrational spectroscopy technique |
| Pôvodný zdroj≠ | Greenfield, N. J., & Fasman, G. D. (1969). Computed circular dichroism spectra for protein secondary structures. Biochemistry, 8(10), 4108-4116. DOI ↗ | Fleischmann, M., Hendra, P. J., & McQuillan, A. J. (1974). Raman spectra of pyridine adsorbed at a silver electrode. Chemical Physics Letters, 26(2), 163-166. DOI ↗ |
| Ďalšie názvy≠ | CD spectroscopy, circular dichroism, CD analysis | Surface-enhanced Raman scattering, SERS spectroscopy |
| Príbuzné | 3 | 3 |
| Zhrnutie≠ | Circular Dichroism (CD) spectroscopy measures the differential absorption of left- and right-circularly polarized light by optically active molecules, particularly proteins and nucleic acids. Introduced by Greenfield and Fasman in 1969, CD is a rapid, non-destructive technique for characterizing secondary structure (alpha-helix, beta-sheet), monitoring protein folding transitions, and assessing conformational changes in response to pH, temperature, or ligand binding. | Surface-Enhanced Raman Spectroscopy (SERS) amplifies weak Raman signals by many orders of magnitude when analyte molecules are adsorbed on specially prepared metal (typically silver or gold) nanostructured surfaces. Discovered by Fleischmann, Hendra, and McQuillan in 1974, SERS enables detection of vibrational signatures of single molecules and ultra-trace contaminants, revolutionizing analytical chemistry and forensics. |
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